RAD-seq of Pacific Oyster: Vancouver Island University - SRA

SRX25641190: RAD-seq of Pacific Oyster: Vancouver Island University
1 ION_TORRENT (Ion Torrent Proton) run: 6.2M spots, 494.9M bases, 345.2Mb downloads

Design: DNA was extracted from adductor muscle sections of Crassostrea gigas, purified and quantified by fluorometry, then normalized to 10 ng/ul and used as an input to double-digest restriction site associated (RAD)-sequencing library preparation. Genomic DNA was digested using NsiI and MspI cutting enzymes, and size selected. Samples were individually barcoded and multiplexed at 32 individuals per chip. Multiplexed libraries were sequenced on an Ion Torrent Proton. Fastq data were trimmed for adapters and low quality sequences, then demultiplexed and truncated to a consistent size (80 bp), then uploaded to NCBI.

Submitted by: Vancouver Island University

Study: Selection of Pacific oysters using an OsHV-1 resistance marker has no influence on susceptibility to Vibrio aestuarianus

PRJNA1145972 • SRP525048 • All experiments • All runs

show Abstracthide Abstract

Reduced representation sequencing (double-digest restriction site-associated DNA sequencing) dataset to support a genome-wide association study of survival to a Vibrio aestuarianus infection in a selectively-bred population of Pacific oyster for viral resistance.

Sample: Not applicable

SAMN43080927 • SRS22285456 • All experiments • All runs

Organism: Magallana gigas

Library:

Name: OFR6.10-Dead_10.28

Instrument: Ion Torrent Proton

Strategy: RAD-Seq

Source: GENOMIC

Selection: Reduced Representation

Layout: SINGLE

Runs: 1 run, 6.2M spots, 494.9M bases, 345.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR30174683	6,186,588	494.9M	345.2Mb	2024-11-21

ID:: 34655767

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